The Comparison of lymphocyte count and PPD test size in children with lymphadenitis caused by BCG vaccination and healthy children

The Comparison of lymphocyte count and PPD test size in children with lymphadenitis caused by BCG vaccination and healthy children

Fatemeh Cheraghali, jalaloddin shaikh,Golestan medical university, Gorgan, Iran

Abstract:

The most common side effects of Bacillus Calmette Guerin (BCG) vaccination is Lymphadenitis. The most common side effects of Bacillus Calmette Guerin (BCG) vaccination is Lymphadenitis. Lymphadenitis that can be seen in 0.1 to 1 percent of the vaccinated individuals. Some immune defects in children affect its prevalence and may increase it. This study designed for measuring lymphocyte count and PPD size in children with Lymphadenitis due to BCG vaccine and normal children.

Methods:

A case-control study was conducted on 40 children (24 males) with lymphadenitis caused by BCG vaccination as well as 40 healthy children (22 males) with no significant medical problem as controls. All participants underwent CBC and PPD test.

Results:

We divided all of the cases based on their size of PPD test into 4 groups (under 5 mm, 5 to 10 mm, 10 to 15 mm, above 15 mm, respectively). In the group of children with lymphadenitis, 21 cases had under 5 mm (52.5%), 14 cases between 5 to 10 mm (35%), 1 cases were 10 to 15 mm (2.5%) and 4 were above 15 mm (10%). In the control group 35 cases under 5 mm (87%), 4 cases were 5 to 10 mm (10%), and 1 case was 10 to 15 mm (3%) and there was no cases greater than 15 mm.The difference was statistically significant according to non-parametric Man-Withney test (P-value = 0.001). Mean PPD induration size was 5.86 mm and 3.04 mm in case and control group respectively with significant difference (P-value=0.004). BCG scar in both groups was in the expected correct site.

The mean lymphocyte count of the study group was 5480 / ml (55.83%) with SD= 2035 and in the control group was 5118 cell / ml (53.93%) with SD = 2793. According to the independent T-test, the difference in mean lymphocyte levels between the two groups was not statistically significant (P-value = 0.51). The mean WBC of the study group was 9810 cell / ml with SD = 2725 and in the control group was 9383 cell / ml with SD = 3425. According to the independent T-test, the mean WBC level between two groups was not statistically significant (P-value = 0.52).

Conclusion:

According to the study it can be concluded that the mean WBC level between two groups was not statistically significant (P-value = 0.52). There is a statistically significant relation between higher incidence of lymphadenitis after BCG vaccination in children with PPD induration size in the cases was higher compared to controls.

Keywords: Lymphadenitis, BCG, PPD, lymphocyte count